Publications

Selected Publications

  • Hartsock, A. and J.P. Shapleigh. 2010. Identification, functional studies, and genomic comparisons of new members of the NnrR regulon in Rhodobacter sphaeroides. J. Bacteriol. 192:903-911.
  • Lee, B. O.M. Usov, V.M. Grigoryants, W.K. Myers, J.P. Shapleigh and C.P. Scholes. 2009. The role of arginine-127 at the proximal NO-binding site in determining hte electronic structure and function of 5-coordinate NO-heme in cytochrome c" of Rhodobacter sphaeroides. Biochemistry 48:8985-8993.
  • Bergaust, L., J. P. Shapleigh, Å. Frostegård, and L. R. Bakken. in press. Transcription and activities of NOx reductases inAgrobacterium tumefaciens: the influence of nitrate, nitrite and oxygen availability. Env. Microbiol.
  • Baek, S. H., A. Hartsock, and J. P. Shapleigh. 2008. Agrobacterium tumefaciens C58 uses ActR and FnrN to control nirK and norexpression. J Bacteriol 190:78-86.
  • Shapleigh, J. P. 2007. The denitrifying prokaryotes, p. 769-792. In M. Dworkin (ed.), The Prokaryotes:A Handbook on the Biology of Bacteria: Volume 2: Ecophysiology and Biochemistry. Springer-Verlag New York, NY.
  • Basaglia, M., A. Toffanin, E. Baldan, M. Bottegal, J. P. Shapleigh, and S. Casella. 2007. Selenite-reducing capacity of the copper-containing nitrite reductase of Rhizobium sullae. FEMS Microbiol Lett 269:124-130.
  • Laratta, W. P., M. J. Nanaszko, and J. P. Shapleigh. 2006. Electron transfer to nitrite reductase of Rhodobacter sphaeroides 2.4.3: examination of cytochromes c2 and cY. Microbiology 152:1479-1488.
  • Casella, S., J. P. Shapleigh, A. Toffanin, and M. Basaglia. 2006. Investigation into the role of the truncated denitrification chain inRhizobium sullae strain HCNT1. Biochem Soc Trans
  • Choi, P. S., Z. Naal, C. Moore, E. Casado-Rivera, H. D. Abruna, J. D. Helmann, and J. P. Shapleigh. 2006. Assessing the impact of denitrifier-produced nitric oxide on other bacteria. Appl Environ Microbiol 72:2200-2205.
  • Usov, O. M., P. S. Choi, J. P. Shapleigh, and C. P. Scholes.2006. ENDOR of NO-ligated cytochrome c'. J Am Chem Soc 128:5021-5032.
  • Usov, O. M., Y. Sun, V. M. Grigoryants, J. P. Shapleigh, and C. P. Scholes. 2006. EPR-ENDOR of the Cu(I)NO complex of nitrite reductase. J Am Chem Soc 128:13102-13111.
  • Baek, S. H., and J. P. Shapleigh. 2005. Expression of nitrite and nitric oxide reductases in free-living and plant-associatedAgrobacterium tumefaciens C58 cells. Appl Environ Microbiol 71:4427-4436.<
  • Choi, P. S., V. M. Grigoryants, H. D. Abruna, C. P. Scholes, and J. P. Shapleigh. 2005. Regulation and function of cytochrome c' inRhodobacter sphaeroides 2.4.3. J Bacteriol 187:4077-4085.
  • Usov, O. M., P. S. Choi, J. P. Shapleigh, and C. P. Scholes. 2005. ENDOR investigation of the liganding environment of mixed-spin ferric cytochrome c'. J Am Chem Soc 127:9485-9494.
  • Baek, S. H., G. Rajashekara, G. A. Splitter, and J. P. Shapleigh. 2004. Denitrification genes regulate Brucella virulence in mice. J Bacteriol 186:6025-6031.
  • Basumallick, L., R. K. Szilagyi, Y. Zhao, J. P. Shapleigh, C. P. Scholes, and E. I. Solomon. 2003. Spectroscopic studies of the Met182Thr mutant of nitrite reductase: role of the axial ligand in the geometric and electronic structure of blue and green copper sites. J Am Chem Soc. 125:14784-14792.
  • Laratta, W. P., and J. P. Shapleigh.2003. Site-directed mutagenesis of NnrR: a transcriptional regulator of nitrite and nitric oxide reductase in Rhodobacter sphaeroides. FEMS Microbiol. Lett. 229:173-178.
  • Yin, S., M. Fuangthong, W. P. Laratta, and J. P. Shapleigh. 2003. Use of a green fluorescent protein-based reporter fusion for detection of nitric oxide produced by denitrifiers. Appl Environ Microbiol 69:3938-3944.
  • Lee, D. Y., A. Ramos, L. Macomber, and J. P. Shapleigh. 2002. The taxis response of various denitrifying bacteria to nitrate and nitrite. Appl. Environ. Microbiol. 68: 2140-2147.
  • Laratta, W. P., P. S. Choi, I. E. Tosques, and J. P. Shapleigh. 2002. Involvement of the PrrB/PrrA two-component system in nitrite respiration in Rhodobacter sphaeroides 2.4.3: evidence for transcriptional regulation. J. Bacteriol. 184:3251-3259.
  • Takada, K., Z. Naal, J.-H. Park, J. P. Shapleigh, S. Bernhard, C. A. Batt, and H. D. Abruña. 2002. Study of specific binding of maltose binding protein to pyrrole-derived bipyridium film using QCM. Langmuir. 18: 4892-4897.
  • Zhao, Y., D. A. Lukoyanov, Y. V. Toropov, K. Wu, J. P. Shapleigh, and C. P. Scholes. 2002. Catalytic function and local proton structure at the Type 2 copper of nitrite reductase: the correlation of enzymatic pH dependence, conserved residues and proton hyperfine structure. Biochemistry. 41:7464-7474.
  • Hosler, J., and J. P. Shapleigh. 2002. Aerobic respiration, Principles of. In G. Bitton (ed.), Encyclopedia of environmental microbiology. Wiley, New York, NY.
  • Bartnikas, T. B., Y. Wang, T. Bobo, A. Veselov, C. P. Scholes, and J. P. Shapleigh. 2002. Characterization of a member of the NnrR regulon in Rhodobacter sphaeroides 2.4.3 encoding a heme-copper protein. Microbiol. 148:825-833.
  • Naal, Z., J.-H. Park, S. Bernhard, J. P. Shapleigh, C. A. Batt, and H. D. Abruña. 2002. Amperometric TNT biosensor based on the oriented immobilization of a nitroreductase maltose binding protein fusion. Anal. Chem. 74:140 -148.
  • Mackenzie, C., M. Choudhary, F. W. Larimer, P. F. Predki, S. Stilwagen, J. P. Armitage, R. D. Barber, T. J. Donohue, J. P. Hosler, J. E. Newman, J. P. Shapleigh, R. E. Sockett, J. Zeilstra-Ryalls, and S. Kaplan. 2001. The home stretch, a first analysis of the nearly completed genome of Rhodobacter sphaeroides 2.4.1. Photosyn. Res. 70:19-41.
  • Jain, R., and J. P. Shapleigh. 2001. Characterization of nirV and a gene encoding a novel pseudoazurin in Rhodobacter sphaeroides2.4.3. Microbiol. 147:2505-2515.
  • Shapleigh, J. P. 2000. The denitrifying prokaryotes. In M. Dworkin (ed.), The prokaryotes: An evolving electronic resource for the microbiological community. Springer-Verlag, New York, NY.
  • Mitchell, D. M., Y. Wang, J. O. Alben, and J. P. Shapleigh. 1998. Fourier transform infrared analysis of membranes of Rhodobacter sphaeroides 2.4.3 grown under microaerobic and denitrifying conditions. Biochim. Biophys. Acta. 1409:99-105.
  • Wu, Q., G. D. Storrier, K. R. Wu, J. P. Shapleigh, and H. D. Abruña. 1998. Electrocatalytic reduction of S-nitrosoglutathione at electrodes modified with an electropolymerized film of a pyrrole derived viologen system and their application to cellular S-nitrosoglutathione determinations. Anal. Biochem. 263:102-112.
  • Olesen, K.O., A. Veselov, Y. Zhao, Y. Wang, B. Danner, C.P. Scholes and J.P. Shapleigh. 1998. Spectroscopic, kinetic and electrochemical characterization of heterologously expressed wild type and mutant forms of copper-containing nitrite reductase from Rhodobacter sphaeroides 2.4.3. Biochem. 37:6086-6094.
  • Veselov, A., K. Olesen, A. Sienkiewicz, J.P. Shapleigh and C.P. Scholes. 1998. Electronic structural information from Q-band ENDOR on the Type 1 and Type 2 copper liganding environment in wild type and mutant products of copper-containing nitrite reductase. Biochem. 37:6095-6105.
  • Wu, Q., G.D. Storrier, F. Pariente, Y. Wang, J.P. Shapleigh and H.D. Abruña. 1997. A nitrite biosensor based on a maltose binding protein nitrite reductase fusion immobilized on an electropolymerized film of a pyrrole derived bipyridinium. Anal Chem. 69: 4856-4863.
  • Kwiatkowski, A.V., W.P. Laratta, A. Toffanin and J.P. Shapleigh. 1997. Analysis of the role of the nnrR gene product in the response of Rhodobacter sphaeroides 2.4.1 to exogenous nitric oxide. J. Bacteriol. 179:5618-5620.
  • Bartnikas, T.B., I.E. Tosques, W.P. Laratta, J. Shi and J.P. Shapleigh. 1997. Characterization of the region encoding the nitric oxide reductase of Rhodobacter sphaeroides 2.4.3. J. Bacteriol. 179:3534-3540.
  • Tosques, I.E., A.V. Kwiatkowski, J. Shi and J.P. Shapleigh. 1997. Characterization and regulation of the gene encoding nitrite reductase in Rhodobacter sphaeroides 2.4.3. J. Bacteriol. 179:1090-1095.
  • Hosler, J.P., J.P. Shapleigh, D.M. Mitchell, Y. Kim, M.A. Pressler, C. Georgiou, G.T. Babcock, J.O. Alben, S. Ferguson-Miller and R.B. Gennis. 1996. Polar residues in helix VIII of subunit I of cytochrome c oxidase influence the activity and the structure of the active site. Biochem. 35:10776-10783.
  • Kwiatkowski, A. and J.P. Shapleigh. 1996. Requirement of nitric oxide for induction of genes whose products are involved in nitric oxide metabolism in Rhodobacter sphaeroides 2.4.3. J. Biol. Chem. 271:24382-24388.
  • Maskus, M., F. Pariente, Q. Wu, A. Toffanin, J.P. Shapleigh and H.D. Abruña. 1996. Electrocatalytic reduction of nitric oxide at electrodes modified with electropolymerized films of [Cr(v-tpy)2]+3 and their applications to cellular NO determinations. Anal. Chem. 68:3128-3124.
  • Mitchell, D.M., J.P. Shapleigh, A.M. Archer, J.O. Alben and R.B. Gennis. 1996. A pH-dependent polarity change at the binuclear center of reduced cytochrome c oxidase detected by FTIR difference spectroscopy of the CO adduct. Biochem. 35:9446-9450.
  • Toffanin, A., Q. Wu, M. Maskus, S. Casella, H.D. Abruña and J.P. Shapleigh. 1996. Characterization of the gene encoding nitrite reductase and the physiological consequences of its expression in the nondenitrifying Rhizobium "hedysari" strain HCNT1. Appl. Environ. Microbiol. 62:4019-4025.
  • Tosques, I.E., J. Shi and J.P. Shapleigh. 1996. Cloning and characterization of nnrR, whose product is required for the expression of proteins involved in nitric oxide metabolism in Rhodobacter sphaeroides 2.4.3. J. Bacteriol. 178:4958-4964.
  • Fetter, J.R., J. Qian, J. Shapleigh, J.W. Thomas, A. Garcia- Horsman, E. Schmidt, J. Hosler, G.T. Babcock, R.B. Gennis and S. Ferguson-Miller. 1995. Possible proton relay pathways in cytochrome c oxidase. Proc. Natl. Acad. Sci. USA. 92:1604-1608.
  • Yun, C.H., B. Barquera, K. Iba, K.I. Takamiya, J. Shapleigh, A.R. Crofts and R.B. Gennis. 1994. Deletion of the gene encoding cytochrome b-562 from Rhodobacter sphaeroides. FEMS Microbiol. Lett. 120:105-110.
  • Hosler, J.P., J.P. Shapleigh, M.M.J. Tecklenburg, J.W. Thomas, Y. Kim, M. Espe, J. Fetter, G.T. Babcock, J.O. Alben, R. Gennis and S. Ferguson-Miller. 1994. A loop between transmembrane helices IX and X of subunit I of cytochrome c oxidase caps the heme a-heme a3-CuB center. Biochem. 33:1194-1201.
  • Garcia-Horsman, J.A., E. Berry, J.P. Shapleigh, J.O. Alben and R.B. Gennis. 1993. A novel cytochrome c oxidase from Rhodobacter sphaeroides that lacks CuA. Biochem. 33:3113-3119.
  • Hosler, J.P., S. Ferguson-Miller, M.W. Calhoun, J.W. Thomas, J. Hill, L. Lemieux, J. Ma, C. Georgiou, J. Fetter, J. Shapleigh, M.M.J. Tecklenburg, G.T. Babcock and R.B. Gennis. 1993. Insight into the active site structure and function of cytochrome oxidase by analysis of site-directed mutants of bacterial cytochrome aa3 and cytochrome bo. J. Bioenergetics and Biomembranes. 25:121-136.
  • Shapleigh, J.P. and R.B. Gennis. 1992. Cloning, sequencing and deletion from the chromosome of the gene encoding subunit I of the aa3-type cytochrome c oxidase of Rhodobacter sphaeroides. Mol. Microbiol. 6:635-642.
  • Shapleigh, J.P., J.J. Hill, J.O. Alben and R.B. Gennis. 1992. Spectroscopic and genetic evidence for two heme-Cu containing oxidases in Rhodobacter sphaeroides. J. Bacteriol. 174:2338-2343.
  • Shapleigh, J.P., J.P. Hosler, M.M.J. Tecklenburg, Y. Kim, G.T. Babcock, R.B. Gennis and S. Ferguson-Miller. 1992. Definition of the catalytic site of cytochrome c oxidase: The specific ligands of heme a and the heme a3-CuB center. Proc. Natl. Acad. Sci. USA. 89:4786-4790.
  • Casella, S., J.P. Shapleigh, F. Lupi and W.J. Payne. 1988. Nitrite reduction in bacteroids of Rhizobium "hedysari" strain HCNT1. Arch. Microbiol. 149:384-388.
  • Shapleigh, J.P., K.J.P. Davies and W.J. Payne. 1987. Detergent inhibition of nitric oxide reductase. Biochim. Biophys. Acta. 911:334-340.
  • Casella, S., J.P. Shapleigh and W.J. Payne. 1986. Nitrite reduction in Rhizobium "hedysari" strain HCNT1. Arch. Microbiol. 146:233-238.
  • Shapleigh, J.P. and W.J. Payne. 1985. Differentiation of c,d1 cytochrome and copper nitrite reductase production in denitrifiers. FEMS Microbiol Lett. 26:275-279.
  • Shapleigh, J.P. and W.J. Payne. 1985. Nitric oxide-dependent proton translocation in various denitrifiers. J. Bacteriol. 163:837-840.
  • Payne, W.J., M.A. Grant, J.P. Shapleigh and P. Hoffman. 1982. Nitrogen oxide reduction in Wolinella succinogenes andCampylobacter species. J. Bacteriol. 152:915-918.